Migrating Peptide Mapping Methods from the Arc™ Premier System to the Alliance™ iS Bio System with Gradient SmartStart Technology and Intelligent Method Translator App (iMTA)

The Alliance iS Bio HPLC System is an innovative biocompatible and exceedingly inert HPLC platform designed for biopharmaceutical manufacturing environments. Through low system dispersion and a default 680 µL mixer, the Alliance iS Bio HPLC System is suitable for biopharmaceutical applications where analytes are subjected to non-specific adsorption to metal surfaces and acidic mobile phase are deployed. 

In this study, we demonstrate how integrated features including Gradient SmartStart Technology and the Intelligent Method Translator App (iMTA) can support seamless transfer of methods from Arc Premier systems with quaternary solvent manager and binary solvent manager configuration while preserving separation selectivity, bypassing the need for time-consuming method re-optimization.

Benefits
- The Intelligent Method Translator App (iMTA) streamlines the method transfer between systems with error free and time saving method translation
- Gradient SmartStart Technology maintains separation selectivity and increases confidence in retention time-based peak assignment
- The Alliance iS Bio HPLC System delivers consistent and reproducible results well-suited for QC environments