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Advancing 3D Metabolism with the Agilent Seahorse XF Flex Analyzer
Credit: Agilent
The Agilent Seahorse XF Flex Analyzer is a next-generation platform for real-time measurement of cellular metabolism, designed to bridge the gap between traditional 2D cultures and complex 3D models such as organoids and tissue slices. By quantifying oxygen consumption rate (OCR) for mitochondrial respiration and extracellular acidification rate (ECAR/PER) for glycolysis, the XF Flex delivers powerful, label-free insights into energy production pathways.
A defining strength of the XF Flex is its optimized support for 3D metabolic workflows. While earlier Seahorse systems excelled with 2D monolayers, the XF Flex integrates enhanced sensitivity and dedicated consumables, such as the XF Flex 3D Capture Microplate-L. This 24-well plate uses precision-engineered capture rings and shallow chambers to hold tissue punches or organoids securely while maintaining gas exchange and reagent access. A tailored mixing protocol ensures stable gradients and robust signal detection, producing consistent results even with variable sample sizes.
The platform’s reproducibility is reinforced by sample preparation protocols, and advanced QC algorithms within Agilent’s software and flexible normalization methods, such as tissue surface area imaging or protein quantification, to account for heterogeneity in 3D samples. These improvements significantly reduce variability, ensuring reliable performance.
By enabling robust metabolic analysis directly in physiologically relevant models, the XF Flex helps scientists better understand disease mechanisms, evaluate therapeutic effects, and reduce reliance on animal models. Its combination of real-time precision, 3D compatibility, and reproducible performance makes it an essential tool for advancing translational research in oncology, neuroscience, drug discovery, and beyond.
The Agilent Seahorse XF Flex Analyzer is a next-generation platform for real-time measurement of cellular metabolism, designed to bridge the gap between traditional 2D cultures and complex 3D models such as organoids and tissue slices. By quantifying oxygen consumption rate (OCR) for mitochondrial respiration and extracellular acidification rate (ECAR/PER) for glycolysis, the XF Flex delivers powerful, label-free insights into energy production pathways.
A defining strength of the XF Flex is its optimized support for 3D metabolic workflows. While earlier Seahorse systems excelled with 2D monolayers, the XF Flex integrates enhanced sensitivity and dedicated consumables, such as the XF Flex 3D Capture Microplate-L. This 24-well plate uses precision-engineered capture rings and shallow chambers to hold tissue punches or organoids securely while maintaining gas exchange and reagent access. A tailored mixing protocol ensures stable gradients and robust signal detection, producing consistent results even with variable sample sizes.
The platform’s reproducibility is reinforced by sample preparation protocols, and advanced QC algorithms within Agilent’s software and flexible normalization methods, such as tissue surface area imaging or protein quantification, to account for heterogeneity in 3D samples. These improvements significantly reduce variability, ensuring reliable performance.
By enabling robust metabolic analysis directly in physiologically relevant models, the XF Flex helps scientists better understand disease mechanisms, evaluate therapeutic effects, and reduce reliance on animal models. Its combination of real-time precision, 3D compatibility, and reproducible performance makes it an essential tool for advancing translational research in oncology, neuroscience, drug discovery, and beyond.
About Agilent Technologies
Agilent Technologies is a global leader in life sciences, diagnostics and applied markets, recognized for uncompromising integrity in all they do. Their mission is to advance quality of life by focusing their expertise in six key markets. With a team of approximately 18,000 employees spread across the world, their global footprint includes sales offices, logistics centers and business facilities in three major geographies. Their worldwide capabilities enable them to deliver high-quality solutions to their valued customers in 110 countries.
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